Slow and ultra-rapid freezing protocols for cryopreserving roe deer (Capreolus capreolus) epididymal sperm collected at different times of year

نویسندگان

چکیده

The roe deer is a monoestrous species with very short rutting season. present work reports the most suitable period for collecting epididymal sperm and describes effect of two cooling rates on post-thaw quality sperm. Testes were collected 24–48 h after death. Samples flushed from epididymis subjected to either (1) dilution in Tris-citric acid-glucose-egg yolk-based medium glycerol, slow freezing straws, or (2) same extender but replacing glycerol 100 mM sucrose, ultra-rapid pellets. Sperm motility, acrosome membrane integrity, morphometry morphological abnormalities analysed before cryopreservation. Spermatogenic activity was investigated via histological examination testis sections. Several testes between April, May September showed no spermatogenic activity. All those June–August No significant difference detected cryoresistance ratios associated conventional freezing, during pre-rutting (April–May) (June–August) periods. differences seen slow-frozen-thawed ultra-rapid-frozen-thawed terms percentage viable abnormalities. Slow returned significantly better (P<0.05) values integrity (43.3% vs. 25.0%) straight-line velocity (19 μm/s 4 μm/s). For both methods, heads smaller post-thawing than pre-freezing (P<0.001). In conclusion, season are periods Ultra-rapid did not provide results.

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ژورنال

عنوان ژورنال: European Journal of Wildlife Research

سال: 2021

ISSN: ['1612-4642', '1439-0574']

DOI: https://doi.org/10.1007/s10344-021-01468-4